| Fr., 23.04.2010 | Tennis Borussia Berlin - FC Oberneuland 1948 | 2:3 |
| Sa., 24.04.2010 | SV Babelsberg 03 - VfB Lübeck | 2:0 |
| Sa., 24.04.2010 | SV Wilhelmshaven - Hannover 96 II | 0:5 |
| Sa., 24.04.2010 | FC Hansa Rostock II - Goslarer SC 08 | 2:1 |
| So., 25.04.2010 | ZFC Meuselwitz - VfL Wolfsburg II | 1:2 |
| So., 25.04.2010 | 1. FC Magdeburg - Chemnitzer FC | 1:0 |
| So., 25.04.2010 | VFC Plauen - Hallescher FC | 4:0 |
| So., 25.04.2010 | Hamburger SV II - Hertha BSC Berlin II | 1:2 |
| So., 25.04.2010 | Türkiyemspor Berlin - FC St. Pauli II | 3:3 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | SV Babelsberg 03 | 30 | 20-8-2 | 47:14 | 33 | 68 |
| 2. | |  | VfL Wolfsburg II | 30 | 19-7-4 | 57:15 | 42 | 64 |
| 3. | |  | Hallescher FC | 30 | 14-12-4 | 44:20 | 24 | 54 |
| 4. | |  | Chemnitzer FC | 30 | 15-7-8 | 54:32 | 22 | 52 |
| 5. | |  | Hamburger SV II | 29 | 12-9-8 | 38:30 | 8 | 45 |
| 6. |  |  | 1. FC Magdeburg | 30 | 12-8-10 | 47:33 | 14 | 44 |
| 7. |  |  | VfB Lübeck | 30 | 13-5-12 | 40:41 | -1 | 44 |
| 8. | |  | Hannover 96 II | 30 | 11-9-10 | 53:37 | 16 | 42 |
| 9. | |  | VFC Plauen | 30 | 11-8-11 | 38:36 | 2 | 41 |
| 10. | |  | ZFC Meuselwitz | 30 | 10-10-10 | 34:42 | -8 | 40 |
| 11. | |  | FC Hansa Rostock II | 30 | 10-6-14 | 34:57 | -23 | 36 |
| 12. | |  | Hertha BSC Berlin II | 29 | 9-7-13 | 46:50 | -4 | 34 |
| 13. | |  | Tennis Borussia Berlin | 30 | 8-9-13 | 27:44 | -17 | 33 |
| 14. | |  | Türkiyemspor Berlin | 30 | 7-9-14 | 40:57 | -17 | 30 |
| 15. | |  | FC St. Pauli II | 30 | 7-9-14 | 32:52 | -20 | 30 |
| 16. | |  | FC Oberneuland 1948 | 30 | 8-6-16 | 34:59 | -25 | 30 |
| 17. | |  | SV Wilhelmshaven | 29 | 7-7-15 | 35:55 | -20 | 28 |
| 18. | |  | Goslarer SC 08 | 29 | 3-8-18 | 28:54 | -26 | 17 |